Finally, combined with the OARSI score of osteoarthritis (Figure 1I; Supplementary Table S2), we can find that rapamycin can indeed reduce the expression of cartilage damage-related factor MMP13, protecting Col2 in cartilage tissue from catabolism factor-induced degradation, thereby delaying the progression of PTOA in ACLT rats. This evidence concerns the gene MMP13 and osteoarthritis.