Sequential presence of IM cells expressing PAX2 on d7, MM expressing SIX2/WT1 on d14 and renal vesicles expressing PAX2/LHX1 on d22 that final differentiated into renal cell types including podocyte-like cells expressing WT1/PODXL/SYNPO, proximal tubular cells expressing CDH1/LTL, distal tubular cells tubules expressing only CDH1 and endothelial cells expressing CD31/vWF+. Off-target neuron-like cells expressing TUJ1 were also observed. Tubules expressed nephron progenitor/renal vesicle markers including LHX1 and PAX2. MM cells expressing SIX2 appear adjacent to organoids. This evidence concerns the gene PECAM1 and Miyoshi myopathy.