Posterior IM cells expressing GATA3/HOXD11 on day 7. Alternatively to organoid generation cells were further differentiated in 2D to UE expressing GATA3/PAX2/ECAD or MM expressing only PAX2 and its derivatives expressing PAX2/ECAD. Preferential induction of UE vs. MM was tested with different extents of CHIR treatment in combination with different growth factors. This evidence concerns the gene HOXD11 and Miyoshi myopathy.