AKT1 and colorectal carcinoma: The result showed that high-dose celastrol (1 μM) reduced or sustained the levels of p-AKT and p-ERK in CRC cells at the 12th h, dependent on each cell line, whilst 0.25 μM celastrol strongly activated AKT and/or ERK signaling across three tested CRC cell lines (Figure 1B; Supplementary Figure S1).