ACTA1 and fibrosis: Immunohistochemical staining also indicated that the expressions of collagen 1 and α-SMA were significantly enhanced in the FAC-treated group compared with those of the erastin and control groups, and under the condition of iron overload, the improved effect of iron on EMs fibrosis was diminished by treatment with Fer-1 and DFO (Figures 5I,J, p < 0.05).