In the current study, incubation of the colon cancer cell line (HCT116) or the non–small–cell lung cancer cell line (A549) with SAF (IC25 or IC50) 24 h prior to TPT increased the amount of DNA double-strand breaks compared to cells treated with TPT alone as indicated by the increased tail moment (Figure 2) and the increased γ–H2AX formation (Figure 3). Here, H2AX is linked to malignant colon neoplasm.