Within this study, implications of β1-integrin-SO/ASO (β1-SO/ASO) and NF-κB-SO/ASO in HCT116 and RKO CRC cells were compared in a multicellular, pro-inflammatory TME in vitro. This 3D-tumor cultures, composed of cancer cells, T-lymphocytes and fibroblasts have been established throughout our previous studies (Buhrmann et al., 2020; Brockmueller et al., 2021) as well as in other research groups (Gao et al., 2021). This evidence concerns the gene NFKB1 and neoplasm.