It has been shown that m5C expression levels and regulators NSUN4, NSUN5, DNMT1, TET2, and ALYREF are significantly upregulated in HCC and are related to poor prognosis in HCC patients (Blanco et al., 2016; He et al., 2020a; He et al., 2020b; He et al., 2020c; Du et al., 2020;Zhang et al., 2020e; Cui et al., 2021b; He et al., 2021; Liu et al., 2022). Here, NSUN4 is linked to hepatocellular carcinoma.