Additionally, through behavioral testing, we analyzed the effect of these different treatment modalities on depression-like symptoms in mice, and by analyzing some physiological indicators of depression (including neurotransmitters, brain-derived neurotrophic factors, inflammatory factors, and antioxidants), receptors in various subregions of the hippocampus, and gut microbiota to explore the corresponding underlying mechanisms. The gene discussed is BDNF; the disease is major depressive disorder.