Additionally, through behavioral testing, we analyzed the effect of these different treatment modalities on depression-like symptoms in mice, and by analyzing some physiological indicators of depression (including neurotransmitters, brain-derived neurotrophic factors, inflammatory factors, and antioxidants), receptors in various subregions of the hippocampus, and gut microbiota to explore the corresponding underlying mechanisms. This evidence concerns the gene BDNF and depressive symptom measurement.