In addition, murine D4M3A melanoma cells (established from the metastatic melanoma Tyr::CreER;BrafCA;Ptenlox/lox mouse model (53)) plated onto brain slices from immunocompetent VE-Cadherin reporter mice (Cdh5-CreERT2;ZSGreenloxp/stop/loxp) mice, only migrated along the pre-existing vasculature of the brain (25, 52). The gene discussed is CDH5; the disease is metastatic melanoma.