To further verify the regulatory role of TGFβ in gastric cancer cells, we cultured gastric cancer cells with conditioned medium incorporating TGFβ1, TGFβ2 and TGFβ3 active proteins and showed that the EMT trend of cancer cells cultured under TGFβ conditions was significantly increased (CDH1 expression was decreased, CDH2, VIM and ZEB1 expression was increased), while inhibition of gastric cancer cells TGFβ1, TGFβ2 and TGFβ3 gene expression suppressed the EMT trend in gastric cancer cells (no significant change in CDH1 expression and decreased expression of CDH2 and ZEB1). Here, ZEB1 is linked to gastric cancer.