Tissue proteins from gastric cancer and normal tissues adjacent to the cancer were extracted, and the results of Western blot experiments showed that the protein expression levels of TGFβ1, TGFβ2 and TGFβ3 were higher in gastric cancer tissues than in normal tissues, which was consistent with the results of transcriptome sequencing levels (GSE184336) (Figure 3B). This evidence concerns the gene TGFB1 and gastric cancer.