DNA methyltransferases DNMT3B, DNMT3A, and DNMT1 were comparatively overexpressed in the MS4A6A high expression subgroup, which might be the reason for MS4A6A hypomethylation in glioma tissues, leading to insight into upregulated mechanisms of MS4A6A in glioma. This evidence concerns the gene DNMT3B and central nervous system cancer.