TLR8 and infection: To further understand the biological roles of immune genes involved in RLR and TLR signaling pathways in anti-IHNV, mRNA levels for IFIH1, DHX58, MAVS, TANK, nucleosome assembly protein 1 (AZI2/NAP1), DDX3X, TLR3, Toll-like receptor 8 (TLR8), MYD88, IRF3, IRF7, and MX1 were analyzed by qRT-PCR during infection (Figure 7).