To verify the accuracy of the transcriptomic data, the 10 hub genes (i.e., CD3D, CD3G, RGS1, CIITA, CYBB, HLA-DQA1, CD69, HLA-DMB, GBP5, and GBP4) were validated by qRT-PCR in nine control individuals and eight childhood asthma patients (Figure 6). The gene discussed is GBP4; the disease is childhood onset asthma.