In our study, miR-335-5p was interlinked with CXCL2, CXCL8 and ICAM1, which led us to speculate that miR-335-5p may alleviate the progression of CD by suppressing the intestinal inflammatory response (CXCL2, CXCL8) and intestinal fibrosis (ICAM1). Here, ICAM1 is linked to Cowden disease.