This study shows that C16orf54 expression in the majority of tumour types was significantly positively linked to immune cell infiltration, including T cell CD4 +, T cell CD8 +, T cell regulatory (Tregs), B-cell, neutrophil, NK cell, monocyte, endothelial cell, macrophage, T cell follicular helper, and myeloid dendritic cell, and significantly negatively correlated with common lymphoid progenitor and eosinophil and myeloid-derived suppressor cells infiltration. This evidence concerns the gene CD4 and neoplasm.