C16orf54 and colorectal carcinoma: C16orf54 expression was discovered to be significantly negatively related to TMB in CHOL, ACC, LUAD, STAD, and BLCA; significantly positively associated with TMB in CRC, COAD, OV, and UCEC (Figure 8(a)); and significantly negatively related to MSI in DLBC, TGCT, ACC, KIPAN, KIRP, GBM, LGG, LUSC, STAD, HNSC, and OV (Figure 8(b)).