Then we analyzed the cell proliferation marker PCNA, autophagy marker LC3-II, the expression of AKT/mTOR, and infiltrates of macrophages (F4/80) and nutrophils (Ly-6G) in tumors form ASH-3 bering mice treated with Salmonella or PBS were analyzed on day 7 and day 14 after infection by immunohistochemistry. This evidence concerns the gene AKT1 and infection.