At the 70% ERα+/ERα−ratio, MDA-MB-231Trans−ER and MDA-MB-231WT cell models showed the strongest cell invasion and migration in vitro as well as the highest M2 macrophage polarization and VEGR, TNF-α, BRCA1, and HER2 expression levels in the tumor microenvironment. Here, TNF is linked to neoplasm.