In this study, we prepared cationic microbubbles (CMBs) loaded with BDNF plasmid (BDNFp) to explore their therapeutic effect with FUS to open the rat BBB and deliver the BDNF gene into the brain the PFC in the autism rat model; the aim of which was to provide new ideas and methods for the clinical treatment of patients with autism. The gene discussed is BDNF; the disease is autism.