Transcriptomic profiling combined with metabolomics, tracer, and flux analysis of mouse B16-F10 tumor ECs (TECs) revealed that these cells rely more on glycolysis than normal ECs (NECs), and glycolytic activator PFKFB3 blockade induces cancer cell invasion, intravasation, and metastasis by normalizing tumor vessels [237]. This evidence concerns the gene PFKFB3 and cancer.