In human lung adenocarcinoma A549 cells, TGF-β treatment-induced EMT, increased the levels of aspartic acid (Asp), Glu, and lysine (Lys), whereas decreased the levels of alanine, asparagine, citrulline), Gln, Gly, histidine, hydroxyproline, isoleucine, Leu, phenylalanine, Pro, threonine, and tyrosine (Tyr). Here, TGFB1 is linked to lung adenocarcinoma.