Initially, we utilised a syngeneic allograft model of pancreatic cancer where C57Bl6/J mice were subcutaneously transplanted with KPC cells (derived from Pdx1-cre;KrasG12D/+;Trp53R172H/+ mice [41, 42]) and therapeutically treated with a single 20 Gy dose of tumour-targeted radiation with or without a SG deficient diet. Here, PDX1 is linked to neoplasm.