Given the link between CHD2 pathogenic mutation and ASD, our findings here provide a set of candidate cis-regulatory elements under the direct control of CHD2 that can be used to further investigate functional interplay between CHD2 and the RFX TFs in regulating neuronal gene expression and how its disruption could contribute to the etiology of ASD and other neurodevelopmental disorders. This evidence concerns the gene RFX1 and neurodevelopmental disorder.