To understand the inflammatory process in the aortic valve under conditions of hyperlipidemia, we performed a flow cytometric analysis of aortic valvular cells, using C57BL/6J, Apoe−/−, and Ldlr−/− mice fed either a chow or WD (Fig. 2a, b and Supplementary Fig. 4). Here, LDLR is linked to hyperlipidemia.