To explore hyperlipidemia-associated cellular dynamics in aortic valves, we sorted total live single cells from the aortic valves of C57BL/6J (wild type), Ldlr−/−, and Apoe−/− mice and performed single-cell transcriptome analysis using a droplet-based 10x Genomics Chromium system (Supplementary Figs. 1b and 6). Here, APOE is linked to hyperlipidemia.