Kaplan–Meier survival analysis of breast patients (TNBC: n = 255; both positive lymph node status and high grade breast cancer: n = 369) showed that lower SPOP expression and higher TWIST1 expression were correlated with poor prognosis in TNBC patients [SPOP: HR 0.64 (0.42~0.98), p-value 0.036; TWIST1: HR 2.1 (1.29~3.42), p-value 0.0023] (Fig. 1G, H), as well as in patients with both positive lymph node invasion and high grades [SPOP: HR 0.65 (0.48~0.88), p-value 0.0056; TWIST1: HR 1.84 (1.32~2.58), p-value 0.00029] (Fig. 1I, J). The gene discussed is SPOP; the disease is breast cancer.