By western blotting analysis, levels of TWIST1 in the majority of cancer tissues were significantly higher than in matched adjacent normal tissues (22 out of 26, Fig. 1D), whereas expression of SPOP in most cancer tissues was much lower than in matched adjacent tissues (21 of 26) (in Fig. 1D labeled blue “N”, Supplementary Fig. S2). The gene discussed is SPOP; the disease is cancer.