EIF4EBP1 and Miyoshi myopathy: Western blot analysis following SLC3A2 knockdown in RAS-dependent MM lines found significant reductions in mTORC1 targets (p-p70S6K (T389) and p-4EBP1 (S65)) but minimal changes in the mTORC2 target PKCα (p-T638/641) (Fig. S3d, e).