We next used the proximity ligation assay (PLA), which can quantitatively visualize protein-protein interactions within tens of nanometers as discrete puncta in situ27, to study interactions between endogenous SLC3A2 and RAS in the KRAS-dependent RPMI 8226 and NRAS-dependent SKMM1 MM cells. The gene discussed is NRAS; the disease is Miyoshi myopathy.