Finally, 180 EOCs (121 serous, 26 mucinous, 8 clear, 25 endometrioid), 42 borderline tumours (35 serous, 7 mucinous), 60 benign tumours (43 serous, 10 mucinous, 7 endometriosis), and 62 nonadjacent normal epithelial tissues for NANOG and 164 EOCs (110 serous, 25 mucinous, 6 clear, 23 endometrioid), 48 borderline tumours (38 serous, 10 mucinous), 73 benign tumours (54 serous, 15 mucinous, 4 endometriosis), and 66 nonadjacent normal epithelial tissues for pAMPK were interpretable for evaluation of the association between NANOG or pAMPK and clinicopathological characteristics in EOC patients. Here, NANOG is linked to benign neoplasm.