Liver fibrosis monitored by the accumulation of α-Sma, collagen I, desmin, and Sirius red staining, as well as by measuring the mRNA levels of α-Sma, Col1a1, Mmp-3, and Tgfβ1, was significantly decreased by KY19334 treatment compared to treatment with selonsertib or ocaliva (Fig. 6i, j and Supplementary Fig. 4a). This evidence concerns the gene COL1A1 and Hepatic fibrosis.