In both the previous14 and new constructs, we use a Rac1 harboring Q61L/P29S mutations (hereafter referred to as Rac1 or Rac1QP interchangeably), in which Q61L stabilizes GTP binding to Rac1, and P29S (an oncogenic mutation identified in melanoma patients22,23) enhances Rac1 binding to WRC14. This evidence concerns the gene RAC1 and melanoma.