To investigate the effect of CAV2 in HNSCC, CAV2 expression was knocked down in SCC15 and SCC25 cells by infection with virus collected from HEK293T cells transfected with a lentivirus-specific expression vector (sh-CAV2-1, sh-CAV2-2), and CAV2 expression was upregulated (CAV2-overexpression; CAV2-OE) by transfection with lentiviral plasmids. The gene discussed is CAV2; the disease is head and neck squamous cell carcinoma.