Next, we measured TERT mRNA by RT-qPCR and like the RNAscope result, TERT was expressed in the tumor tissue but not in the adjacent “normal” FFPE punches and greater than or equal to FFPE punches obtained from a separate glioblastoma case with the hotspot mutation G228A (Fig. 5e). The gene discussed is TERT; the disease is neoplasm.