Notably, Ub diGly sites representing K63- and K48-linked chains were decreased in both LCLs (RPS27A_K63 in V335M; RPS27A_K48 in D628V) which is different from reports describing elevated Ub K48 levels in cells expressing the ALS-linked Cyclin-F mutation S621G (34). The gene discussed is CCNF; the disease is amyotrophic lateral sclerosis.