Based on these results, to further explore the effect of ACACB on CRC cell growth and cetuximab resistance in CRC cell lines, two shRNAs were synthesized to knock downACACB, and stable knockdown cell lines (KD#1 and KD#2) were generated according to the manual in HCT116, SW620 and NCI-H508 cell lines (Figure 4C). The gene discussed is ACACB; the disease is colorectal carcinoma.