To obtain cell lines in which the NME1 or NME2 genes were completely and stably inactivated, we performed CRISPR–Cas9 gene editing in the human breast tumor cell line MCF10DCIS.com using two independent guide RNAs specific to the NME1 gene, NME1 (#A) and NME1 (#B), and two for the NME2 gene, NME2 (#A) and NME2 (#B). Here, NME1 is linked to breast neoplasm.