TWIST2 and neoplasm: In the present study, a series of target genes of miR-22-5p were predicted, as miR-22-5p could regulate the migration, proliferation, and EMT, the target of miR-22-5p must be associated to tumor malignant progression, and then we clarified that miR-22-5p could directly target TWIST2, a transcription factor associated with tumor malignant progression [28].