Here, we expressed interest in whether CDK6 has a biological function as the downstream target of ZFP36, and for further validation, we found CDK6 at mRNA level and protein levels in PCa cells and tumor xenografts with enhanced and depleted ZFP36 expression decreased and increased, respectively, demonstrating the negative regulatory effect of ZFP36 on CDK6, which was further confirmed using luciferase reporter and RNA coimmunoprecipitation assays. This evidence concerns the gene ZFP36 and posterior cortical atrophy.