In a study of Alzheimer’s Disease, researchers found that SIRT3 deficiency caused microglia activation following exposure to a combination of high glucose and palmitic acid using SIRT3 shRNA Lentiviral particles in BV2 cells, and enhanced microglial and endothelial interactions which may leading to BBB breakdown, and thus caused cognitive dysfunction, however, the mechanism of SIRT3 deficiency under microglial and endothelial interactions has not been further exacerbated (Tyagi et al., 2021). Here, SIRT3 is linked to Alzheimer disease.