In Kaplan–Meier analysis with the log-rank test, PD-L1 expression did not significantly affect survival in stage II and III colon cancer analyzed together (PD-L1-positive: mean DFS 51.7 months (95% CI 47.4-55.9) vs. PD-L1-negative mean DFS 48.6 months (46.5-50.7), p=0.120) (Supplementary Figure) or in subgroup analyses. Here, CD274 is linked to malignant colon neoplasm.