Probiotic supplementation in combination with interval training significantly decreased tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) mRNA and matrix metalloproteinase-2(MMP-2) mRNA in the liver (p<0.05), while the levels of lysosomal acid lipase(LIPA) mRNA was significantly increased compared to NAFLD group. This evidence concerns the gene MMP2 and metabolic dysfunction-associated steatotic liver disease.