The insomnia-related targets related to insomnia were screened based on data obtained from the Genecards (https://www.genecards.org/) and OMIM (https://omim.org/) databases, and a total of 2,934 targets including synuclein alpha, interleukin 6, delayed sleep phase syndrome, proline dehydrogenase 1, and restless legs syndrome were collected (Supplementary Table S2). The gene discussed is PRODH; the disease is restless legs syndrome.