To further verify the accuracy of our bioinformatic analysis results, we verified the mRNA and protein levels of the hub FRGs using RT-qPCR and IHC, respectively, and we found that there were only four hub FRGs, including Il1b, Ptgs2, Cybb, and Cd44 that were significantly differentiated in IH induced by carotid artery ligation. Here, IL1B is linked to isolated hemihyperplasia.