Upregulation-trends of Ptgs2 in ferroptosis was further demonstrated in the study by Zhou et al. In this study, they found that the expression of PTGS2, ACSL4, caspase-1, and NLRP3 were upregulated at the late stages of atherosclerosis, and these proteins could be used as biomarkers of atherosclerosis severity (Zhou and Zhou, 2021). This evidence concerns the gene ACSL4 and atherosclerosis.