In this study, AraC-loaded liposomes with ALN and HA as dual-targeting ligands on the surface were successfully developed by postinsertion manner, and the liposomes decorated with ALN-HA on the surface via a bio-reducible linker (-SS-) could achieve bone site and leukemia cells targeting in turn, our experiment revealed that the formulation had the strong affinity to HAP in vitro and accumulation capacities of bone-targeting in vivo. This evidence concerns the gene ARLN and leukemia.