Among these four proteins, PFDN5α was further taken for validation to confirm its reactivity to B-ALL CSF using both 1D western and ELISA platform based on its potential role in controlling one of the genes involved in leukemia pathogenesis, the c Myc at different levels such as (a) repressing transcriptional activity of c-Myc, (b) promotes degradation of c-Myc, (c) transcriptional repression of Wnt4 involved in the Wnt-β-catenin pathway that activate c-Myc expression (Table S3)16,17. The gene discussed is MYC; the disease is acute lymphoblastic leukemia.