We compared the immune microenvironment of combo and control 4T1 tumours and found a significant increase in Ki67+ CD8+ T cells in combo-treated tumours that were infiltrated throughout the tumour with an increase in CD8+/Treg ratio following combinational treatments, as determined by manual gating analyses and reflect the induction of an effective immune response by inosine in combination with ICB (Supplementary Fig. 3n). Here, CD8A is linked to neoplasm.