Using a previously validated flow cytometry strategy for CAF subtypes [3, 6], we evaluated myCAFs (PDPN+Ly6C−MHCII−), iCAFs (PDPN+Ly6C+MHCII−), and apCAFs (PDPN+Ly6C−MHCII+) located within either hypoxic (Hypoxyprobe+) or normoxic (Hypoxyprobe−) tumor regions (Fig. 1E). The gene discussed is PDPN; the disease is neoplasm.