NUCLEOLIN and breast cancer: To probe the linkage between NCL-meditated SOCE and the proliferation of breast cancer cells, we established the stable breast cancer cell lines (MCF-7 and MDA-MB-231) with constitutive overexpression of NCL and performed MTT assay (Fig. 5A), colony formation (Fig. 5B) and EdU proliferation assays (Fig. 5C, D).