Of the 100 nonsynonymous CYP8B1 variants (Supplemental Table 1), only 1 (R26X) was both a CLOF mutation (0.6% activity compared with wild-type CYP8B1), common in our study cohort (1.6% mutation frequency in Malays, Genome Aggregation Database [gnomAD v2.1.1, https://gnomad.broadinstitute.org/] allele frequency = 8.58 × 10–5), and present in several copies in the AMP-T2D-GENES data set. This evidence concerns the gene CYP8B1 and type 2 diabetes mellitus.