BRAF and melanoma: In contrast to Braf/Pten mice, pigmented lesions developed very slowly on ears and dorsal skin of Tyr:CreERT2(tg/0): BrafLSL–V600E/+ (Braf mice), appearing 2 months after Tam injection or topical 4-HT treatment with melanomas barely observed within 6 months, as previously reported (16).