Since we had already found decreased M2 cytokines and chemokines in the tumor lysates, and increased T cell proliferation with TAMs from APR-246–treated mice, we investigated the transcriptomic consequences of p53-regulated genes in TAMs after APR-246 therapy in sorted TAMs (CD45+CD11b+TCRβ–F4/80+) by quantitative real-time polymerase chain reaction (RT-PCR) (Figure 5D). Here, TP53 is linked to neoplasm.