Triple combination treatment efficacy is CD8-dependent. Radiation alone reduced neutrophilic myeloid-derived suppressor cells and increased Treg tumour accumulation, unchanged with the addition of AZD1775. T-cells from tumour-draining lymph nodes (TDLNs) from mice treated with the triple therapy demonstrated the greatest activation and IFNγ production upon exposure to MOC1 tumour antigen. Mice cured following triple agent treatment did not engraft tumours following rechallenge. The gene discussed is IFNG; the disease is neoplasm.