AZD6738 further increased RT-stimulated CD8+ T cell infiltration and activation and reverted the immunosuppressive effect of radiation on the number of Tregs in mice xenografts. Triple combination with anti-PD-L1 boosted the infiltration, cell proliferation, enhanced IFN-γ production ability of TIL CD8+ T cells, decreased trend in number of TIL Tregs and exhausted T cells in mice xenografts. Triple therapy led to more long-lasting immunity with tumour rechallenge rejection. This evidence concerns the gene CD8A and neoplasm.