A prior study had demonstrated let7g-5p as a tumor-suppressive miRNA associated with impaired GSC behavior.67 Bioinformatic analysis predicted that let7g-5p shares a binding site with mitogen-activated protein kinase 1 (MAP3K1), a protein previously shown to correlate directly with shortened patient survival and increased TMZ resistance in GBM cells.68 Subsequent mRNA results showed that MAP3K1 expression was significantly increased in GBM cells transfected with let7g-5p shRNA. This evidence concerns the gene MAP4K1 and glioblastoma.