ZO-1 and occludin, the most crucial tight junction proteins (Camilleri et al., 2012), interact directly with actin (Camilleri et al., 2012), and their expressions are lower in IBS patients (Bertiaux-Vandaële et al., 2011) as well as mice (Hou et al., 2019). The gene discussed is OCLN; the disease is irritable bowel syndrome.